Device

Part:BBa_K165100:Design

Designed by: John Szymanski   Group: iGEM08_BrownTwo   (2008-10-29)


Gli1 bs + LexA bs + mCYC + LexA repressor (mCherryx2 tagged) on pRS304*


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 534
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 534
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2587
    Illegal XhoI site found at 1
    Illegal XhoI site found at 122
    Illegal XhoI site found at 284
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 534
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 534
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 119
    Illegal SapI.rc site found at 1758
    Illegal SapI.rc site found at 2351


Design Notes

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Source

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References